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where does glycolysis take place

Glycolysis takes place in the cytoplasm of the cell, in both prokaryotes and eukaryotes.

Location in the cell

  • In eukaryotic cells (like human cells), glycolysis occurs in the cytoplasm, not in the mitochondria.
  • In prokaryotic cells (like bacteria), it also occurs in the cytoplasm because they lack membrane-bound organelles.

What happens there

  • One glucose molecule in the cytoplasm is broken down into two pyruvate molecules, producing a net gain of 2 ATP and 2 NADH.
  • This is the first step of cellular respiration and can proceed without oxygen (it is an anaerobic process).

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