In many itch.io games, you can turn off audio from the main menu or settings with an option labeled Sound, Audio, or Volume. For the specific case of a game that includes audio description, one itch.io post says to press A or F1 on the main screen to disable it.

Try these first

  1. Open the game’s main menu and look for Settings , Options , or a speaker icon.
  2. Set Music , SFX , or Master Volume to zero.
  3. If the game has audio description , press A or F1 on the title screen.
  1. In browser games, also check whether audio starts only after a click, since some itch.io games use autoplay restrictions.

If there is no menu

Some itch.io games simply do not include an in-game mute toggle, and then the fastest workaround is your browser or device volume control. A few games expose sound toggles only after you begin play, while others hide them in a pause menu or a small icon in a corner.

Quick note

If you meant a game with a built-in audio description feature, the direct shortcut above is the clearest fix I found.