In a wild-type E. coli cell, housekeeping genes (such as those encoding RNA polymerase core subunits, ribosomal RNAs, ribosomal proteins, and enzymes of central metabolism like glycolysis) are constitutively expressed.

Key idea

  • Constitutive expression means the gene is transcribed at a relatively constant level, independent of specific environmental inducers or repressors.
  • In wild-type E. coli , this applies mainly to essential ā€œhousekeepingā€ functions , not to typical inducible operons like lac or trp , which are tightly regulated by nutrients (lactose, tryptophan, etc.).

Common exam-style options

When this question appears in genetics or microbiology exams, the constitutively expressed choice is usually something like:

  • A gene encoding RNA polymerase subunit (e.g., rpoB/rpoC)
  • A ribosomal RNA gene (rRNA, e.g., rrn operons)
  • A core ribosomal protein gene
  • A central metabolism enzyme (e.g., glycolytic enzyme)

Whereas genes like lacZ , trpE , or other catabolic/biosynthetic operon genes are not constitutive in a wild-type strain; they are induced or repressed depending on substrate or amino acid availability.

How to pick the answer

  • If the options include a clear housekeeping gene (e.g., ā€œrRNA geneā€, ā€œRNA polymerase geneā€, ā€œgene for a ribosomal proteinā€) alongside regulatory/inducible genes (lac , trp , stress-response), choose the housekeeping gene as the constitutively expressed one.

TL;DR: In a wild-type E. coli , the constitutively expressed gene among typical choices is the one coding for a basic housekeeping function (rRNA, ribosomal protein, or RNA polymerase), not lac or trp operon genes.

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