what kind of analysis is needed to determine whether two mutations are in the same gene or in different genes?
The kind of analysis used is a complementation test (also called a complementation analysis).
Quick Scoop
To figure out whether two mutations are in the same gene or in different genes, classical genetics uses a complementation test, where organisms carrying each mutation are crossed and the phenotype of the offspring is examined.
If the offspring show a wildâtype (normal) phenotype, the mutations complement and are interpreted as being in different genes, because each mutationâs defect is ârescuedâ by a functional copy from the other parent.
If the offspring still show the mutant phenotype, the mutations fail to complement and are interpreted as being in the same gene (different alleles of that gene), because no functional copy of that gene is present in the hybrid.
In short: use a complementation test to determine whether two mutations are in the same gene or in different genes.
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