The most likely answer is Anime World Tower Defense. In 2023, it was one of the main anime tower defense games on Roblox, and it’s commonly listed among the early/major titles in that genre.

Why this fits

  • “Second anime tower defense” is usually referring to the second big Roblox anime TD game people remember after the original wave.
  • Anime World Tower Defense is a well-known Roblox title and has a dedicated wiki entry identifying it as a Roblox game.
  • Later discussion and ranking videos still group it among the core anime tower defense games on Roblox.

Other possible meaning

If you meant the second part of a YouTube series or a specific game title with “#2” in it, that would be different. One search result is a Roblox Studio development video labeled “Anime Tower Defense #2,” but that is a tutorial episode, not the game name itself.

Context note

If your question is about the second major anime tower defense game released in 2023 , that exact ranking isn’t clearly standardized across sources, so the safest answer is Anime World Tower Defense as the likely intended game.