Glycolysis occurs in the cytoplasm (cytosol) of cells, in virtually all living organisms.

Core answer

  • In both prokaryotic and eukaryotic cells, glycolysis takes place in the cytoplasm, not inside mitochondria or other organelles.
  • It is the first stage of cellular respiration and happens whether or not oxygen is present (aerobic and anaerobic conditions).

Quick Scoop

  • Location: Cytoplasm/cytosol of the cell.
  • Applies to: Nearly all cell types across all domains of life.
  • Role: Breaks one glucose (6‑carbon) into two pyruvate (3‑carbon) molecules while producing a small amount of ATP and NADH.

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