Chromosomes line up in the middle of the cell during metaphase , specifically along an imaginary line called the metaphase plate.

Quick Scoop

  • The stage of the cell cycle where chromosomes arrange themselves across the middle of the cell is metaphase of mitosis.
  • In metaphase, spindle fibers attach to each chromosome and pull them so they line up along the metaphase plate, a central plane equidistant from both poles of the cell.
  • A common memory trick students use is “M for middle = metaphase,” since that is when chromosomes sit in the center before being separated to opposite sides of the cell.

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