In anaphase 2 of meiosis, the sister chromatids of each chromosome split apart at the centromere and are pulled to opposite poles of the cell by spindle fibers. This ensures each future gamete gets one copy of each chromosome.

Quick Scoop: what happens in anaphase 2

Think of anaphase 2 as the “final pull-apart” step before gametes are formed:

  1. Sister chromatids separate
    • The centromeres divide, so each chromatid becomes an individual chromosome.
    • This is different from anaphase 1, where whole homologous chromosomes separate, not chromatids.
  2. Chromatids move to opposite poles
    • Spindle fibers attached to kinetochores shorten and drag the chromatids toward opposite ends of the cell.
    • Non-kinetochore microtubules may lengthen, helping stretch the cell.
  3. Chromosome number stays haploid
    • Cells were already haploid after meiosis 1 (one of each homologous pair).
    • Anaphase 2 just splits the duplicated chromosomes, so each pole still has a haploid set, but now with single (undoubled) chromosomes.
  4. Sets up telophase 2 and four gametes
    • Once the chromatids reach the poles, telophase 2 starts, nuclei reform, and cytokinesis produces four genetically unique haploid cells.

Simple way to remember:
Anaphase 1 = homologous chromosomes separate
Anaphase 2 = sister chromatids separate

TL;DR: In anaphase 2, the centromeres split, sister chromatids separate, and are pulled to opposite poles, preparing the cell to form four haploid gametes with one copy of each chromosome.